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  • 產(chǎn)品名稱(chēng)： Anti-GATA3 antibody [A3G3]
  • 產(chǎn)品貨號(hào)： CS1902-33
  • 貨期： 現(xiàn)貨
  • 價(jià)格與訂購(gòu)： 1500
  • 數(shù)量：
    庫(kù)存： 100
  • 規(guī)格： 50μl 100μl
  • 產(chǎn)品信息
  • 如何訂購(gòu)
    產(chǎn)品描述
    GATA3 is a transcription factor that in humans is encoded by the GATA3 gene. Studies in animal models and humans indicate that it controls the expression of a wide range of biologically and clinically important genes. The GATA3 transcription factor is critical for the embryonic development of various tissues as well as for inflammatory and humoral immune responses and the proper functioning of the endothelium of blood vessels. GATA3 haploinsufficiency (i.e. lose of one or the two inherited GATA3 genes) results in a congenital disorder termed the Barakat syndrome. Current clinical and laboratory research is focusing on determining the benefits of directly or indirectly blocking the action of GATA3 in inflammatory and allergic diseases such as asthma. It is also proposed to be a clinically important marker for various types of cancer, particularly those of the breast. However, the role, if any, of GATA3 in the development of these cancers is under study and remains unclear.
    產(chǎn)品名稱(chēng)
    Anti-GATA3 antibody [A3G3]
    分子量
    Predicted band size: 48 kDa.
    種屬反應(yīng)性
    Human
    驗(yàn)證應(yīng)用
    WB,ICC,FC
    抗體類(lèi)型
    小鼠單抗
    免疫原
    Synthetic peptide within N-terminal Human GATA3.
    偶聯(lián)
    Non-conjugated
    形態(tài)
    Liquid
    濃度
    2 mg/ml.
    存放說(shuō)明
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
    存儲(chǔ)緩沖液
    1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
    亞型
    IgG2c
    純化方式
    Protein A affinity purified.
    亞細(xì)胞定位
    Nucleus.
    數(shù)據(jù)鏈接
    SwissProt: P23771 Human
    其它名稱(chēng)
    GATA 3 antibody        GATA binding factor 3 antibody          GATA binding protein 3 antibody
    應(yīng)用
    WB: 1:500-1:2,000          ICC: 1:50-1:200           FC: 1:50-1:100
     
    Fig1: Western blot analysis of GATA3 on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Specific bands were detected for GATA3 full length (FL) at approximately 52 kDa and the splice form (SF) at approximately 39 kDa (as indicated).
    Fig2: ICC staining of GATA3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1902-33, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor?488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
    Fig3: Flow cytometric analysis of GATA3 was done on jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-33, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
    背景文獻(xiàn)
    1. Lin MC et al. GATA3 interacts with and stabilizes HIF-1α to enhance cancer cell invasiveness. Oncogene 36(30):4243-4252 (2017).
    Note
    For research use only .