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  • 產(chǎn)品名稱： Biotin Alkyne
  • 產(chǎn)品貨號： CSC303
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 900
  • 數(shù)量：
    庫存： 10
  • 規(guī)格： 5mg
  • 產(chǎn)品信息
  • 如何訂購
    Introduction
    Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
    The biotin alkyne is reactive with azide via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin? biotin-binding protein.
    Features
    Efficiency—the click reaction is complete in less than 1 hour;
    Specificity—the reaction between the label and detection tag is selective and specific;
    Stability—the reaction product contains an irreversible, covalent bond;
    Biologically inert—the components of the reaction do not undergo any side reactions.
    Figure 1. Click chemistry labeling
    Specifications
    Label
    Biotin  
    Ex/Em
    – 
    Detection Method
     – 
    Solubility
    DMSO, DMF 
    Molecular Weight
    413.53 
    Product Size
    5 mg 
    Storage Conditions
    -20 ℃, protect from light 
    Shipping Condition
    Room Temperature 
    Reference
    1.Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
    Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,J Am Chem Soc (2008) 130:11576-11577
    2.Robust fluorescent detection of protein fatty-acylation with chemical reporters.
    Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,J Am Chem Soc (2009) 131:4967-4975
    3.Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification.
    Dias WB, Cheung WD, Wang Z, Hart GW,J Biol Chem (2009) 284:21327-21337
    Note
    For research use only .