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  • 產(chǎn)品名稱： Andy Fluor™ 594 DBCO
  • 產(chǎn)品貨號(hào)： CSC333
  • 貨期： 現(xiàn)貨
  • 價(jià)格與訂購(gòu)： 2500
  • 數(shù)量：
    庫(kù)存： 10
  • 規(guī)格： 0.5 µmol
  • 產(chǎn)品信息
  • 如何訂購(gòu)
    Introduction
    Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
    The Andy Fluor? 594 DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC).
    Features
    Efficiency—the click reaction is complete in less than 1 hour;
    Specificity—the reaction between the label and detection tag is selective and specific;
    Stability—the reaction product contains an irreversible, covalent bond;
    Biologically inert—the components of the reaction do not undergo any side reactions.
    Figure 1. Click chemistry labeling
    Label
    Andy Fluor? 594   
    Ex/Em
    590/615 
    Detection Method
    Fluorescent 
    Solubility
    DMSO, DMF 
    Product Size
    0.5 μmol 
    Storage Conditions
    -20 ℃, protect from light 
    Shipping Condition
    Room Temperature
    Applications
    Click chemistry labeling 
    Reference
    1.Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
    Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,Angew Chem Int Ed Engl (2012) 51:3143-3146
    2.Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.
    Winz ML, Samanta A, Benzinger D, J?schke A,Nucleic Acids Res (2012) 40:e78-e78
    Note
    For research use only .