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  • 產(chǎn)品名稱： Anti SMO(A68V) Mouse Monoclonal Antibody
  • 產(chǎn)品貨號(hào)： CST26425
  • 貨期： 現(xiàn)貨
  • 價(jià)格與訂購(gòu)： 4800
  • 數(shù)量：
    庫(kù)存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購(gòu)
    Catalog Number
    CST26425
    Gene Symbol
    SMO; MRD21
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    Description
     Anti SMO(A68V) Mouse Monoclonal Antibody
    Background
    moothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
    Immunogen
    A synthetic peptide from the internal region of SMO which includes the mutation of A68V, human origin.
    Applications
    ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes A68V mutant, but not wild-type SMO of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant SMO(A68V) and wild-type proteins. Purified His-tagged SMO(A68V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti SMO(A68V) monoclonal antibody (Cat. #26425).
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    Immunofluorescence: 

    Immunofluorescence of cells expressing SMO proteins with Anti SMO(A68V) antibody. HEK293T cells were transfected with pCDNA3-GFP SMO(WT) plasmid (left column) or pCDNA3-GFP SMO(A68V) plasmid (right column), then fixed and stained with Anti SMO(A68V) monoclonal antibody (Cat. #26425).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.