瀏覽量: 44
- 產(chǎn)品名稱: Recombinant Human COIL protein
- 產(chǎn)品貨號: CSP01044
- 貨期: 現(xiàn)貨
- 價格與訂購: 1800
- 數(shù)量:
- 規(guī)格: 50μg 100μg 1mg
- 產(chǎn)品信息
- 如何訂購
概述(Summary)
英文全稱
Recombinant Human COIL protein
純度(Purity)
>90% as determined by SDS-PAGE
內(nèi)毒素(Endotoxin level)
Please contact with the lab for this information.
蛋白構(gòu)建(Construction)
A DNA sequence encoding the human COIL (Ser319-Glu538) was fused with His tag
Accession #
P38432
表達宿主(Host)
E.coli
種屬(Species)
Homo sapiens (Human)
預(yù)測分子量(Predicted Molecular Mass)
24.85 kDa
制劑(Formulation)
Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
運輸方式(Shipping)
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
穩(wěn)定性&儲存(Stability &Storage)
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
復(fù)溶(Reconstitution)
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
背景(Background)
背景介紹
The enzyme BirA is a key reagent because of its ability to biotinylate proteins at a specific residue in a recognition sequence. We have used this enzyme to biotinylate the C termini of membrane proteins, allowing these proteins to be tetramerized by binding to streptavidin. Because of the specificity of the biotinylation at the C terminus, the orientation of the membrane proteins on the streptavidin is equivalent to that of the native protein on the cell surface. These tetrameric proteins can be used to study protein receptor-ligand interactions at the cell surface, and site-specific biotinylation can be used to study proteins in vitro using a defined orientation. The biotinylation of histones by BirA ligase is consistent with the proposed role of human HCS in chromatin. The N-terminal BirA domain is required for both transcriptional regulation of biotin synthesis and biotin protein ligase activity.
分子別名(Alternative Names)
Coilin,p80-coilin.
Note
For research use only .