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  • 產(chǎn)品名稱: Anti-DNA-RNA Hybrid [S9.6] Antibody
  • 產(chǎn)品貨號: CS2112
  • 貨期: 現(xiàn)貨
  • 價(jià)格與訂購: 2800
  • 數(shù)量:
    庫存: 100
  • 規(guī)格: 100μl
  • 產(chǎn)品信息
  • 如何訂購
    Synonyms
    DNA-RNA hybrid; RNA/DNA   hybrid 
    Attribute
    Mouse   Monoclonal   Antibody
    Isotype
    Mouse IgG2a
    Purity
    Protein A/G Purification
    Application
    ELISA, IP, IF, CHIP, Dot Blot 
    Immunogen
    ΦX174 bacteriophage-derived synthetic DNA/RNA
    Buffer
    PBS with 0.1% sodium azide and 50% glycerol,   pH 7.2 
    Storage
    Store at -20℃.   Do not aliquot
    Recommended Dilution
    IP: 1:50-500IF: 1: 200 
    Background
    The DNA-RNA   hybrids are a natural occurrence within eukaryotic cells and their level are   high at sites of high transcriptional activity. They are non-canonical   nucleic acid structures with transcriptional regulatory functions. Their   presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal   antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind   to single or double stranded DNA. The antibody has high affinity for DNA-RNA   hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of   the antibody appears to be determined by a combination of sequence and   structural dependency since R-loop sequence affects binding affinity. 
     
         
    Immunofluorescentanalysis of ( 4% PFA) fixed HeLa cells using  DNA-RNA hybrid Mouse Monoclonal   Antibody [S9.6]   at dilution of 1:500  and Alexa Fluor 647- conjugatedAffiniPure Goat Anti-Mouse IgG(H+L)
    Chromatin   immunoprecipitationanalysis of   HeLa cells
    genomic DNA(gDNA)using DNA-RNA hybrid Mouse Monoclonal   
    Antibody [S9.6]   at dilution of 1:200
    Note
    For research use only .