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概述（Summary）

產(chǎn)品描述（Description）

ATG fect solution 是一種基于陽離子聚合物的優(yōu)異轉(zhuǎn)染試劑。它可應(yīng)用于多種細(xì)胞系的各種規(guī)模的轉(zhuǎn)染。與市場上其他的轉(zhuǎn)染試劑相比，ATG fect solution 能夠高效率轉(zhuǎn)染多種細(xì)胞系，包括貼壁細(xì)胞和懸浮細(xì)胞，尤其對于懸浮細(xì)胞（如 293-F、CHO-S 等）表現(xiàn)出卓越的性能，同時也具有具有較低的細(xì)胞毒性。 ATGfect solution 是用于瞬時和穩(wěn)定轉(zhuǎn)染的重組蛋白生產(chǎn)的理想選擇。

儲存條件（Storage）

請于-20 °C 保存，保質(zhì)期一年。

運(yùn)輸方式（Shipping）

藍(lán)冰運(yùn)輸

Note

For research use only .

狀態(tài)（Form）

Liquid

使用方法（Standard Operating Procedure）

PROTOCOL FOR SUSPENSION CELLS:
Preparation: 18 to 24 hours before transfection, seed cells at 1.0 x 106 per mL of culture. Sample Transfection.Protocol (1000 mL culture in 3L shake flask).

1. Prepare ATG fect solution-DNA transfection mixture (order is critical):
(1) Add 500 μg of plasmid DNA into100 mL FBS-free medium.
(2) Briefly mix/vortex solution.
(3) Add 1.5 mL of ATGfect solution to mixture.
(4) Vortex solution for 5 seconds.
(5) Let solution sit for 20 minutes in hooded environment to allow ATG fect solution -DNA complexes to form.
(6) Gently mix solution by pipetting up and down 3 times.

2. Add entire transfection solution to 1000 mL of cell suspension culture.

3. Return cell suspension to incubator.

4. Recombinant protein can be analysed 4-6 days after transfection.

Note: The above operation is for reference only. Please explore the ratio of transfection reagent to DNA according to your experimental needs to find the best transfection conditions.